TABLE 1.

P. aeruginosa strains and plasmids used in this study

Strain or plasmidGenotype or characteristicsaSource or referenceb
Strains
    P. aeruginosa strains
        WTWT P. aeruginosa PAO1; H103Lab collection
        UW WTUW WT P. aeruginosa PAO1UW (15)
        UW-psrApsrA::ISlacZ/hah-Tcr; insertion at position 46 (702 bp) in psrA; derived from UW WTUW (15)
        psrA mutantpsrA::ISlacZ/hah-Tcr; H103 background; TcrThis study
        psrA (Tn7-psrA+)psrA mutant with Tn7-psrA+ integrated; Tcr GmrThis study
        PA14Wild-type P. aeruginosa PA1422
        coxA05_2::A11; derived from PA1422
        etfA04_4::A12; derived from PA1422
        fhp09_1::F11; derived from PA1422
        mexC01_4::H2; derived from PA1422
        pprB08_3::C3; derived from PA1422
        rhlG05_3::A8; derived from PA1422
        flp14_1::F4; derived from PA1422
        rcpA01_2::B12; derived from PA1422
        tadA01_2::A7; derived from PA1422
        tadB04_2::H5; derived from PA1422
        wbpM03_4::E4; derived from PA1422
        wzz06_1::F2l; derived from PA1422
        PA1883 (homolog)12_1::A7; derived from PA1422
        wbpIwbpI::ISlacZ/hah-Tcr; insertion 807 (1,065 bp)15
        wbpLwbpL::ISlacZ/hah-Tcr; insertion 302 (1,020 bp)15
    E. coli strain
        TOP10F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
Plasmids
    pCR-Blunt II-TOPOPCR cloning vector; KanrInvitrogen
    pCR-psrA+pCR-BluntII-TOPO harboring 1.12-kb psrA ampliconThis study
    pUC18-mini-Tn7T-GmSuicide plasmid; Gmr AmprH. Schweizer (8)
    pUC-Tn7-psrA+pUC18-mini-Tn7T-Gm with 1.12-kb psrA fragment from pCR-psrAThis study
    pTNS2Transposition helper plasmid; AmprH. Schweizer (8)
  • a Antibiotic resistance phenotypes are indicated as follows: Ampr, ampicillin resistance for E. coli and carbenicillin resistance for P. aeruginosa; Gmr, gentamicin resistance; Kanr, kanamycin resistance; Tcr, tetracycline resistance.

  • b UW, University Washington.