TABLE 3.

Type III secretion system, adhesion (tad), motility, and type II secretion genes significantly dysregulated in psrA mutants, determined using a microarray

Gene identifieraGene nameChange (fold)bP valueDescription
Type III secretion genes
    PA0044exoT4.60.0005Exoenzyme T; type III secretion system effector
    PA1695pscP3.0<0.0001Translocation protein in type III secretion
    PA1697pscN2.10.004ATP synthase in type III secretion system
    PA1698popN2.60.004Outer membrane protein PopN
    PA16992.30.003Conserved hypothetical protein in type III secretion
    PA17002.00.0009Conserved hypothetical protein in type III secretion
    PA17014.50.0003Conserved hypothetical protein in type III secretion
    PA17022.00.0002Conserved hypothetical protein in type III secretion
    PA1703pcrD2.00.01Type III secretory apparatus protein PcrD
    PA1705pcrG3.80.002Regulator in type III secretion
    PA17062.10.001Type III secretion protein PcrV
    PA1707pcrH2.40.0002Regulatory protein PcrH
    PA1708popB3.10.005Translocator protein PopB
    PA1709popD2.70.002Translocator protein PopD
    PA1710exsC2.1<0.0001Exoenzyme S synthesis protein C
    PA1712exsB2.00.001Exoenzyme S synthesis protein B
    PA1715pscB2.50.001Type III export apparatus protein
    PA1717pscD2.80.01Type III export protein PscD
    PA1721pscH2.00.01Type III export protein PscH
    PA1723pscJ2.10.01Type III export protein PscJ
    PA2191exoY2.10.0004Adenylate cyclase ExoY; type III secretion effector
    PA3841exoS2.60.001Exoenzyme S; type III secretion effector
    PA3842orf13.50.001Chaperone for ExoS secretion
Adhesion and motility genes
    PA0176aer2−4.20.04Aerotaxis methyl-accepting chemotaxis protein
    PA1803lon−2.00.05ATP-dependent Lon protease
    PA4296pprB−4.9*0.05PprB two-component response regulator
    PA4300tadC−2.20.04Flp pilus assembly protein, PilC-like
    PA4302tadA−4.1*0.01TadA traffic ATPase in Flp pilus assembly
    PA4303tadZ−2.70.02Flp pilus assembly protein
    PA4305rcpC−2.10.05Flp pilus assembly protein
Type II secretion genes
    PA0683hxcY−4.40.008Hxc type II secretion system membrane protein
    PA1871lasA10.50.002LasA protease
    PA2672hplW−2.50.003Type II secretion system prepilin peptidase substrate
  • a According to the P. aeruginosa genome website (http://www.pseudomonas.com/).

  • b Regulation of genes differentially expressed in the psrA mutant relative to the WT. A positive number indicates transcript upregulation in the psrA mutant. *, confirmation of gene regulation by qRT-PCR.