TABLE 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionTransposon insertiona (position in genome)Source or reference
Strains
    S. coelicolor
        M145Prototrophic; SCP1 SCP231
        DSCO2085-1M145 ftsW::Tn5062(1)SC4A10.2.H05 (2239002)This study
        DSCO2085-2M145 ftsW::Tn5062(2)SC4A10.1.H10 (2238747)This study
        DSCO2085-3M145 ftsW::Tn5062(3)SC4A10.2.F03 (2238425)This study
        DSCO2085-4M145 ftsW::Tn5062(4)SC4A10.2.F08 (2238210)This study
        DSCO2085-5M145 ftsW::Tn5062(5)SC4A10.1.F05 (2237893)This study
        DSCO2083M145 ftsQ::Tn5062SC4A10.2.B05 (2236542)This study
        DSCO2090M145 ftsI::Tn5062SC4A10.1.C09 (2246734)This study
        DSCO2607M145 sfr::Tn5062SCC88.2.H10 (2830098)This study
        DSCO3847M145 SCO3847::Tn5062SCH69.1.B04 (4232371)This study
        DSCO5302M145 SCO5302::Tn5062SC6G9.1.D07 (5775697)This study
        DSCO3846::pSHRC1M145::pSHRC1 rodA::Tn5062SCH69.1.E11 (4230660)This study
        DSCO5302/2607M145 SCO5302::Tn5062 SCO2607::Tn5062-hygSC6G9.1.D07 (5775697), SCC88.2.H10 (2830098)This study
        DSCO2085/2607M145 ftsW::Tn5062 SCO2607::Tn5062-hygSC4A10.2.H05 (2239002), SCC88.2.H10 (2830098)This study
        DSCO2085/5302M145 ftsW::Tn5062-hyg SCO5302::Tn5062SC4A10.2.H05 (2239002), SC6G9.1.D07 (5775697)This study
    E. coli
        JM109F′ traD36 proA+B+ lacIq Δ(lacZ)M15 Δ(lac-proAB) glnV44 e14 gyrA96 recA1 relA1 endA1 thi hsdR1752
        ET12567(pUZ8002)dam13::Tn9 dcm-6 hsdM hsdR recF143 zjj-201::Tn10 galK2 galT22 ara14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtlI glnV44, containing the nontransmissible oriT mobilizing plasmid pUZ800221
Plasmids
    pBluescript II SK(+)Cloning vector, ampicillin resistance1
    pKF41ftsZ::EGFP26
    pSH152E. coli-S. coelicolor shuttle vector, hygromycin resistanceThis study
    pSHBW1pSH152 containing ftsW, murG, and ftsQThis study
    pSHBW4pSH152 containing the ftsW gene and part of murDThis study
    pSHBW7pSH152 with ftsQThis study
    pSHBW12pSHBW1 with in-frame deletion in ftsWThis study
    pSHRC1pSH152 containing SCO3843 to SCO3847This study
    pSFTI1pSH152 containing SCO2092, ftsL and ftsIThis study
    pSFTI3pSH152 containing SCO2093, SCO2092, ftsL, and ftsIThis study
    pUWL219E. coli-S. coelicolor shuttle vector, ampicillin and thiostrepton resistance51