TABLE 1.

Host strains and plasmids used or generated in this study

Strain or plasmidDescriptionReference or source
pE9034AETEC strain E9034A virulence plasmid; carries Longus, CS3, ST, and LT genes13
E. coli DH5αsupE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1Laboratory collection
E. coli HB101supE44 Δ(mcrC-mrr) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1Laboratory collection
pBluescript SKIICloning vector (Apr)Stratagene, La Jolla, CA
pCR2.1Cloning vector (Apr Kmr)Invitrogen, Carlsbad, CA
pACYC184Cloning vector (Cmr Tetr)New England BioLabs, Ipswich, MA
pJAG15.0-kb BamHI fragment containing Longus genes cloned into pACYC17711
pOG140pBluescript SK with 1.0-kb EcoRV insert containing entire lngA gene13
pOG341pACYC184 with 8-kb SalI insert containing lngF to lngPThis study
pOG347pACYC184 with 5-kb EcoRI insert containing lngR to lngAThis study
pOG349pACYC184 with 3-kb EcoRI insert containing lngG to lngHThis study
pOG378pACYC184 with 3-kb EcoRI insert containing lngT to lngBThis study
pOG380pACYC184 with 5-kb NcoI insert containing lngJ to lngPThis study
pOG381Same as pOG380 but with inverted 5-kb NcoI insert containing lngJ to lngPThis study
pOG371pCR2.1 with 1.5-kb lngR-lngS insertThis study