TABLE 2.

Calculated kinetic parameters for M. tuberculosis IspDa

SubstrateKm (μM)Vmax (pmol/min)kcat (min−1)kcat/Km (mM−1 min−1)
MEP58.5 ± 5.428.0 ± 0.730.7212.3
CTP53.2 ± 4.538.6 ± 0.871.018.8
  • a Each reaction mix contained 38.5 pmol of M. tuberculosis IspD. The Km and Vmax values were calculated from averaged data from three independent experiments, using nonlinear regression analysis (GraFit 5.0.13).