TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant propertiesSource or reference
E. coli strains
    BL21(DE3)F ompT hsdSB(rB mB) gal dcm (DE3)Novagen
    DH5αF φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Laboratory strain
    One shot TOP10F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
    S17-1 λpirrecA thi pro hsd(R M+) RP4 2-Tc::Mu-Km::Tn7 λpir32
Y. pestis strains
    KIM5-3001pCD1+, pMT1+, pPCP1+, pgm7
    KIM8-3002pCD1+, pMT1+, pPCP1, pgm7
    GY5475KIM5-3001, crp::pEP185.2This study
    GY5476KIM5-3001, cyaA::pEP185.2This study
    GY5477KIM8-3002, crp::pEP185.2This study
    GY5478KIM8-3002, cyaA::pEP185.2This study
    GY5523KIM5-3001, crp::pEP185.2, pWSK129This study
    GY5524KIM5-3001, crp::pEP185.2, pGY776This study
    GY5531KIM5-3001, pGY778This study
    GY5533KIM5-3001, crp::pEP185.25, pGY778This study
    GY5538KIM5-3001, crp::pEP185.2, pWSK129, pGY778This study
    GY5539KIM5-3001, crp::pEP185.2, pGY776, pGY778This study
    GY5564KIM5-3001, cyaA::pEP185.2, pGY778This study
    GY5585KIM5-3001, cyaA::pEP185.2, pWSK129This study
    GY5586KIM5-3001, cyaA::pEP185.2, pGY803This study
    GY5595KIM5-3001, cyaA::pEP185.2, pWSK129, pGY778This study
    GY5597KIM5-3001, cyaA::pEP185.2, pGY803, pGY778This study
    GY5744KIM5-3001, pGY878This study
    GY5745KIM5-3001, pGY879This study
    GY5746KIM5-3001, pGY880This study
Plasmids
    pCR-Blunt II-TOPOpUC ori, KanrInvitrogen
    pEP185.2mob+, oriR6K, Cmr15
    pET24b(+)KanrNovagen
    pRW50oriV, lacZYA, Tetr19
    pWSK129pSC101 ori, Kanr36
    pGY606pCR-Blunt II-TOPO with a 0.4-kb internal fragment of cyaAThis study
    pGY609pEP185.2 with a 0.4-kb internal fragment of cyaAThis study
    pGY725pCR-Blunt II-TOPO with a 0.3-kb internal fragment of crpThis study
    pGY735pEP185.2 with a 0.3-kb internal fragment of crpThis study
    pGY775pCR-Blunt II-TOPO with a 1.1-kb fragment containing crpThis study
    pGY776pWSK129 with a 1.1-kb fragment containing crpThis study
    pGY777pCR-Blunt II-TOPO with a 0.242-kb fragment containing the pla promoterThis study
    pGY778pRW50 with a 242-bp fragment containing the pla promoterThis study
    pGY802pCR-Blunt II-TOPO with a 3.0-kb fragment containing cyaAThis study
    pGY803pWSK129 with a 3.0-kb fragment containing cyaAThis study
    pGY809pET24b(+) with a 0.6-kb fragment containing crpThis study
    pGY811pCR-Blunt II-TOPO with a 0.6-kb fragment containing crpThis study
    pGY875pCR-Blunt II-TOPO with a 206-bp fragment containing the pla promoterThis study
    pGY876pCR-Blunt II-TOPO with a 206-bp fragment containing the pla promoter with two point mutations altering the cAMP-Crp binding siteThis study
    pGY877pCR-Blunt II-TOPO with a 185-bp fragment containing the pla promoter missing the cAMP-Crp binding siteThis study
    pGY878pRW50 with a 206-bp fragment containing the pla promoter fused to lacZYAThis study
    pGY879pRW50 with a 206-bp fragment containing the pla promoter with two point mutations altering the cAMP-Crp binding site that is fused to lacZYAThis study
    pGY880pRW50 with a 185-bp fragment containing the pla promoter missing the cAMP-Crp binding site that is fused to lacZYAThis study