TABLE 1.

Strains and plasmids used in this work

Strain or plasmidDescriptionReference or source
Strains
    V. fischeri
        ES114Wild-type E. scolopes light organ isolate4
        CL21ainS::cam24
        CL42luxO::kan24
        CL53luxR::erm22
        PMF8litR::kan14
        VCW2G7luxI (frameshift)24
        MB21124acs::TnermThis study
        KV909rpoS::ermK. Visick
        JB24Δcrp5
    E. coli
        DH5α-λpirCloning vector17
Plasmids
    pCL112ainS in a 2.1-kb fragment in pVO8 with an Erm resistance cassette24
    pAKD701pVSV209 with a promoterless lacZ cassette and a Kan resistance cassetteA. Dunn; 12
    pSVS101pAKD701 with a V. fischeri acs promoter region inserted in front of promoterless lacZThis study
    pVO8pACYC with Cam and Erm resistance cassettes46
    pMF2litR in a 1-kb fragment in pVO8 with an Erm resistance cassette14