TABLE 3.

In vivo concentrations of NTP and ppGppa

Stress treatmentStrain genotype (condition)bConcn (mM) of:
GTPATPCTPUTPppGpp
HPLCrelA+ (+K)1.37 ± 0.302.33 ± 0.630.60 ± 0.121.25 ± 0.25NDc
relA+ (−K)0.23 ± 0.127.13 ± 1.11.53 ± 0.561.55 ± 0.210.067 ± 0.020
relA1 (+K)1.26 ± 0.312.14 ± 0.480.47 ± 0.091.02 ± 0.15ND
relA1 (−K)1.09 ± 0.398.51 ± 2.121.81 ± 0.222.50 ± 0.12ND
relA+ (−Dc)1.24 ± 0.413.40 ± 1.20.78 ± 0.151.57 ± 0.51ND
relA+ (+Dc)0.36 ± 0.225.30 ± 1.91.37 ± 0.300.83 ± 0.15ND
relA1 (−Dc)0.71 ± 0.243.04 ± 1.020.49 ± 0.080.80 ± 0.12ND
relA1 (+Dc)0.25 ± 0.185.50 ± 0.451.20 ± 1.201.70 ± 0.35ND
CE/MSrelA+ (+K)0.35 ± 0.152.15 ± 1.080.32 ± 0.060.81 ± 0.15ND
relA+ (−K)0.089 ± 0.04211.94 ± 2.501.07 ± 0.071.04 ± 0.350.027 ± 0.015
relA+ (−Dc)0.44 ± 0.163.13 ± 1.240.38 ± 0.051.19 ± 0.08ND
relA+ (+Dc)0.19 ± 0.037.88 ± 2.020.75 ± 0.111.29 ± 0.05ND
  • a The in vivo concentrations of NTP and ppGpp in cells of strains 1A765 (relA+) and 1A766 (relA1) 30 min after stress treatment were determined as described in the text.

  • b +K and −K denote without and with lysine starvation, respectively. −Dc and +Dc denote without and with decoyinine treatment, respectively.

  • c ND, not detected.