TABLE 1.

Strains and plasmids used in this study

Strain or plasmidCharacteristic(s)Reference or source
Strains
    Vibrio anguillarum
        M93SmSpontaneous Smr mutant of M93 (serotype J-O-1) 6
        M99Smr Cmr empA mutant; pNQEmpA insertion into empA 7
        MSD1Smr Kmr epp mutant; mini-Tn10Km insertion into epp This study
        M101Smr Cmr epp mutant; pMV07 insertion into epp This study
        M102Smr Cmr Tcr; M101 complemented with pSUP202-epp This study
    Escherichia coli
        DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK +) phoA supE44 λ thi-1 gyrA96 relA1 Invitrogen
        XL1MRF′ recA1 endA1 gryA96 thi-1 hsdR17 supE44 relA1 (lac -pro) [F′ proABlacI lacZΔM15 Tn10 (Tcr)]Stratagene
        SM10 thi thr leu tonA lacY supE recA RP4-2-Tc::Mu::Km (λpir) 16
        CC118λpir pLOFKm 13
Plasmids
    pBluescript SKII+ Apr lacZ; pUC ORIStratagene
    pNQ705-1Suicide vector, requires pir ; Cmr 16
    pSUP202Shuttle vector; Tcr Apr Cmr GenBank accession no. AY428809; 7
    pMV01pBSK+ + MSD1 mini-Tn10Km fragment; Apr Kmr This study
    pMV07pNQ705 + epp fragment in SacI/XbaI site; Cmr This study
    pSUP202-epp epp inserted into Apr cassette of pSUP202; Cmr Tcr This study