Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant genotype or commentaSource or reference
Strains
    S. coelicolor
        M600SCP1 SCP225
        J3359ΔcdgB::apr SCP1 SCP2This work
    E. coli
        ET12567/pUZ8002ET12567 containing helper plasmid pUZ800229
Plasmids
    pMS82Plasmid cloning vector for the conjugal transfer of DNA from E. coli to Streptomyces spp., integrated site specifically at the ΦBT1 attachment site (Hygr)15
    pIJ10257Plasmid integrating at the ΦBT1 attB attachment site of S. coelicolor and containing the strong ermEp* promoter (Hygr)21
    pDONR207Gateway donor vectorInvitrogen
    pHNGWAGateway T7 system-based cloning vector for protein overexpression3
    pIJ10346pUC19 carrying cdgB driven by its native promoterThis work
    pIJ10347pMS82 carrying cdgB driven by its native promoterThis work
    pIJ10350pIJ10257 carrying cdgBThis work
    pIJ10352pIJ10257 carrying cdgB-FLAGThis work
    pIJ10361Like pIJ10352 but carrying the GGC1-to-GCG mutationThis work
    pIJ10362Like pIJ10352 but carrying the GGC2-to-GCG mutationThis work
    pIJ10370pHNGWA carrying cdgBThis work
    pIJ10371Like pIJ10370 but carrying the GGC1-to-GCG mutationThis work
    pIJ10372Like pIJ10370 but carrying the GGC2-to-GCG mutationThis work
  • a GGC1 and GGC2, the first and second GGC codons, respectively, of the conserved GGDEF motif.