TABLE 2.

Primers used for PCR or RT-PCR

PrimerSequencea
NorBF5′-CCCGAATTCTAATAACAGCACTTGGTATAC
NorBR5′-CCCGAATTCAAAAACCAATCCCTATGTTC
Tet38F5′-CCCGGATCCGTATGGGTATTTTAGGTGTA
Tet38R5′-CCCGGATCCCGTGTTATATAATCTCTTTA
TET-75′-AGGTATGGGGATCCCATCTATTGG
TET-85′-CCTGTTGAAGTTTTAACGAAGTCCG
SANB-35′-GCTATCATCATGATGTCAATGGGAG
SANB-45′-CTTAATAATAGACAAAGTTGCAGGC
SANB-55′-AAGTAGGCGTTGCTGCAGGTATC
SANB-65′-CTGTTTAACCACGCTTGCTGGTTG
norA-RTf5′-ATCGGTTTAGTAATACCAGTCTTGC
norA-RTr5′-GCGATATAATCATTTGAGATAACGC
norB-RTf5′-ATGGAAAAGCCGTCAAGAGA
norB-RTr5′-AACCAATGATTGTGCAAATAGC
norC-RTf5′-ATGAATGAAACGTATCGCGG
norC-RTr5′-GTCTGCACCAAAACTTTGTTGTAAA
tet38-RTf5′-AAATTGATTTCTGTAGCCATTGCTG
tet38-RTr5′-ACAGCGCCAATACCAATTACT
mgrA-RTf5′-GAACAGCTATGCTTTAGTTTGTACA
mgrA-RTr5′-GACAAGAAATTGTGGGTATGTTAGA
gmk-RTf5′-TATCAGGACCATCTGGAGTAGG
gmk-RTr5′-CATCAACTTCACCTTCACGC
attRf5′-GCTGTCTTTTTCGTGACATTCA
attRr5′-TTTGAAATCCAGGAAGTCCAC
attLf5′-CTGCCTATGATTTAACGTTAGATG
attLr5′-GCAGTTACAGAGTGGAATAGAGCAT
  • a Appended nucleotides with restriction endonuclease sites are underlined.