TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionSource or referencea
Strains
    DH5αλ φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA hsdR17 (rk mk) supE44 thi-1 gyrA relA117
    BL21(DE3)F ompT hsdSB (rB mB) gal dcm (DE3)40
    DHB4Δ(ara-leu)7697 araD139 ΔlacX74 galE galK rpsL phoR Δ(phoA) pvuII ΔmalF3 thi7
    GJ8025DHB4 ΔtrxA grxA::kan zbi::Tn10
Plasmids
    pET23aPT7-based expression vectorNovagen
    pBAD33l-Arabinose-based expression vector with p15A origin of replication15
    pSCM1101pET23a bearing trxAMtb (Rv1470) within NdeI/HindIII restriction sites
    pSCM1102pET23a bearing trxBMtb (Rv1471) within NdeI/HindIII restriction sites
    pSCM1103pET22b bearing trxCMtb (Rv3914) within NdeI/HindIII restriction sites
    pSCM1104pET23a bearing trxAEc within NdeI/HindIII restriction sites
    pSCM1105pET23a bearing trxRMtb (Rv3913) within NdeI/HindIII restriction sites
    pHYD3058pBAD33 bearing trxAMtb within XbaI/HindIII restriction sites
    pHYD3059pBAD33 bearing trxBMtb within XbaI/HindIII restriction sites
    pHYD3060pBAD33 bearing trxCMtb within XbaI/HindIII restriction sites
    pHYD3064pBAD33 bearing trxAEc within XbaI/HindIII restriction sites
  • a Unless otherwise indicated, the source of the listed plasmids is the present study.