TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or characteristicsaReference(s) or source
S. aureus strains
    RN4220 (r-)Restriction-deficient mutagenized RN450 12
    MW2CA-MRSA (USA400 lineage) 1, 3
    MW2 norB MW2 norB::cat This study
    MW2 tet38 MW2 tet38::cat This study
    MW2 geh::pSK950MW2 with pSK950 insert in geh This study
    MW2 norB geh::pSK950MW2 norB with pSK950 insert in geh This study
    MW2 tet38 geh::pSK950MW2 tet38 with pSK950 insert in geh This study
    MW2 norB geh::pSKnorB MW2 norB with pSKnorB insert in geh This study
    MW2 tet38 geh::pSKtet38 MW2 tet38 with pSKtet38 insert in geh This study
E. coli DH5αGeneral host for pUC vectorGibco-BRL
Plasmids
    pSK950Spr (E. coli), Tcr and Emr (S. aureus), temperature sensitive, L54a attP site 18
    pYL112Δ19Apr (E. coli) Cmr (S. aureus), pYL112 without attP site 13, 31
    pCL52.2Tcr, E. coli-S. aureus shuttle vector, temperature sensitive 23
    pYO1pCL52.2 with norB::cat fragmentThis study
    pYO2pCL52.2 with tet38::cat fragmentThis study
    pSKnorB pSK950 with norB complementary fragment, temperature sensitiveThis study
    pSKtet38 pSK950 with tet38 complementary fragment, temperature sensitiveThis study
    pUC19Apr, general-purpose cloning vector 30
  • a Ap, ampicillin; Cm, chloramphenicol; Em, erythromycin; Sp, spectinomycin; Tc, tetracycline.