TABLE 1.

Purification of FRD from H. thermophilus

FractionTotal activitya (U)Total protein (mg)Sp acta (U/mg)Purification factor (fold)Yield (%)
CFE1,3003,8000.351.0100
Butyl-Toyopearl6002302.67.546
DEAE-Toyopearl250347.52119
Hydroxyapatite1103.630878.5
MonoQ390.77501403.0
Phenyl Superose3.50.049712000.27
  • a FRD activity was measured using NADH as an electron donor.