TABLE 3.

Redox potentials of M. tuberculosis thioredoxins and other oxidoreductasesa

ProteinEo (mV)
M. tuberculosis thioredoxin A (TrxAMtb)−248 ± 3
M. tuberculosis thioredoxin B (TrxBMtb)−262 ± 2
M. tuberculosis thioredoxin C (TrxCMtb)−269 ± 2
E. coli thioredoxin A (TrxAEc)−278 ± 3
E. coli glutaredoxin (4)−233
T4 bacteriophage thioredoxin (22)−230
  • a The redox potentials of M. tuberculosis thioredoxins and other prototype members of the thioredoxin family are shown. The equilibrium constant and redox potential (Eo) values for TrxAMtb, TrxBMtb, TrxCMtb, and TrxAEc were obtained using equations 1, 2, and 3 as described in the text. Known redox potentials for some members are denoted as reported previously (4, 23). In the current experiments, the redox potential of TrxA was recorded at −280 mV, close to that reported by Åslund et al. (4).