TABLE 4.

Catabolite repression by the mutant HPrK/P-G158A protein lacking phosphorylase activity

Carbon sourceaEnzyme activityb in U/mg of protein (SD) in strain:
168c (wild type)GP858(pGP380) (ΔhprK)GP858(pGP650) [ΔhprK hprK(G158A)]
None945 (281)2,077 (323)178 (10)
Ribose497 (138)NG115 (30)
Arabinose414 (136)1,175 (50)138 (27)
Maltose437 (127)1,265 (82)142 (17)
Sucrose126 (20)1,232 (145)124 (66)
Gluconate116 (11)822 (97)63 (6)
Sorbitol114 (20)990 (52)90 (22)
Glycerol82 (15)NG70 (15)
Mannitol72 (13)1,592 (460)60 (8)
Fructose60 (9)1,364 (355)64 (8)
Salicin54 (6)981 (168)46 (27)
Glucose7 (3)870 (11)21 (4)
  • a That is, added to the CSE medium.

  • b The values are the averages of at least two independent experiments. NG, no growth.

  • c Xylose was added to induce xynPB expression.