TABLE 1.

B. subtilis strains used in this study

StrainGenotypeSource or referencea
168trpC2Laboratory stock
GP270trpC2 xylR::ermCpIW11xylR→168
GP278trpC2 xylR::ermC amyE::(xynP-lacZ cat)48
GP279trpC2 xylR::ermC crh::spc amyE::(xynP-lacZ cat)QB7097→GP278
GP284trpC2 xylR::ermC ptsH1pIW11xylR→QB5223
GP287trpC2 xylR::ermC ptsH1 crh::spcpIW11xylR→QB7101
GP289trpC2 xylR::ermC hprK::aphA3QB7160→GP270
GP297trpC2 xylR::ermC crh::spcQB7097→GP270
GP853trpC2 xylR::ermC ccpA::spcQB5407→GP270
GP858trpC2 ΔhprK::aphA3QB7160→168
GP864trpC2 ΔptsI::ermCpGP811→168
QB5223trpC2 ptsH130
QB5407trpC2 ccpA::spc9
QB7097trpC2 crh::spcI. Martin-Verstraete
QB7101trpC2 ptsH1 crh::spcI. Martin-Verstraete
QB7144trpC2 amyE::(xynP-lacZ cat)11
QB7160trpC2 amyE::(levD-lacZ cat) hprK::aphA329
  • a Arrows indicate construction by transformation.