TABLE 2.

Kinetic parameters of M. tuberculosis thioredoxinsa

ProteinKm (μM)kcat (s−1)kcat (s−1)/Km (M)
TrxAMtbNDNDND
TrxBMtb2.65 ± 0.071.79 ± 0.13(6.7 ± 0.03) × 105
TrxCMtb2.05 ± 0.51.17 ± 0.24(5.75 ± 0.02) × 105
TrxAEc2.8 ± 0.141.34 ± 0.12(4.7 ± 0.06) × 105
  • a The assay was carried out as described in Materials and Methods. The apparent Km and apparent turnover (kcat) values were calculated by nonlinear regression using the Michaelis-Menten equation. Data are represented as means ± standard deviations. ND, not detected.