TABLE 2.

Pyocyanin production

StrainPyocyanin production (μM) in strain carryinga:
pEX1.8pZOE01
PAO142.6 ± 8.3284.4 ± 19.3
PJF-QE1ND276.5 ± 18.2
PJF-QA1ND261.5 ± 29.7
PJF-QR1ND233.6 ± 44.3
PDO111NDND
PDO100ND15.3 ± 6.5
  • a Data are the means ± SDs of results from at least three independent experiments. pEX1.8, control plasmid; pZOE01, PqsE overexpression plasmid. ND, not detectable. Strains PJF-QA1 and PJF-QR1 produced 34.3 ± 8.4 and 60.8 ± 5.6 μM pyocyanin, respectively, when complemented by plasmids containing a wild-type copy of the gene that had been mutated.