TABLE 2.

Oligonucleotides used in this study

PrimerSequence (5′to 3′)Use
virT-FGAAGGTAAGTACCAAGATGANorthern blotting
virT-RTGATATTGCCACCCCAACTTNorthern blotting
ccp-FGAGGCCGAAAAGACTGAAGGNorthern blotting
ccp-RGCCCAATGTGGTATTGCTTGNorthern blotting
virU-FCGATTCGTTTTTGATAGAAATGGNorthern blotting
virU-RTTTATTTCTAGTTTTTCCTTTGATGANorthern blotting
vrr-FGACCAGTTACGCACAAACNorthern blotting
vrr-RGGACAGTTCTATTTCTAGGNorthern blotting
pfoA-FGCAAGTATTGCAATGGCTTTNorthern blotting
pfoA-RGTAAGTAATACTAGATCCAGGGTNorthern blotting
colA-FGGTCTAGAGGATAGTGGAAGANorthern blotting
colA-RGTTCCTCTCATATCGTAAGTNorthern blotting
0845-PETTTATCAATGGGTAACGTAAAGAAAGTACCAGATAPrimer extension
0846-PEGTTGAAACTCCTCCTAATAGAACGCTACCAATGGTPrimer extension
0920-PETCGGTATAATAAAAATGATATTGTTACTAATATGPrimer extension
virT-NMGAAAGTACCAGATTAAATAGAAATAGSite-directed mutagenesis
virU-NM-1TTGGTATTATATATTAATATTTTCGTCSite-directed mutagenesis
virU-NM-2GACGAAAATATTAATATATAATACCAASite-directed mutagenesis
virT-FM-FATCGCTTTAAGTTTATCATTTCTATTTTATCTGGTSite-directed mutagenesis
virT-FM-RACCAGATAAAATAGAAATGATAAACTTAAAGCGATSite-directed mutagenesis
virU-FM-FGATATGAAAGACGAAAAATTAATATAAAATACCAASite-directed mutagenesis
virU-FM-RTTGGTATTTTATATTAATTTTTCGTCTTTCATATCSite-directed mutagenesis