TABLE 2.

Frequencies of mutation to streptomycin resistance and complementation with pUCPMS for isolates from patient 1

StrainFrequency of mutation to streptomycin (1,000 μg/ml) resistanceComplementation with pUCPMSa
PAO19.1 × 10−10 ± 2.6 × 10−10
PAOMAb5.6 × 10−10 ± 0.6 × 10−10
PAOMSb1.2 × 10−7 ± 0.7 × 10−7+
PAOMSAb2 × 10−7 ± 0.2 × 10−7+
1-0.5 (6 mo)9.8 × 10−10
1-8 (96 mo)7.6 × 10−8+
  • a Complementation was considered positive when the mutation frequency after transformation of pUCPMS was decreased ≥2 logs.

  • b PAOMA is a mucA mutant of PAO1, PAOMS is a mutS mutant of PAO1, and PAOMSA is a mutS mucA mutant of PAO1.