TABLE 1.

Bacterial strains and plasmids used in this study

Strain(s) or plasmidDescription or relevant characteristicsReference or source
E. faecalis strains
    JH2-2Fusr Rifr; plasmid-free wild-type strain30
    ΔglpK mutantJH2-2 with a 190-bp central deletion in glpK introducing a frameshift mutationThis work
    ΔgldA1 mutantJH2-2 with a 635-kbp deletion in gldA1 introducing a frameshift mutationThis work
    ΔgldA1ΔglpK mutantDouble mutant with the aforementioned deletions in the glpK gene and gldA1 operonThis work
    TX0104Information and (partial) genome sequence available from the BCM databaseaB. E. Murray, unpublished data
    MMH594Information and (partial) genome sequence available from the BCM database27
    HH22Information and (partial) genome sequence available from the BCM database22
    ATCC 19433American Type Culture Collection
    V583Genome sequence available from TIGRb25
    OG1RFGenome sequence available from the BCM database10
    EF CI isolates 1 to 9Nine clinical isolates from various backgroundsJ. M. Entenza and J. Huebner, unpublished data
E. coliTop10F′Invitrogen
Plasmids
    pGEM-T EasyPromega
    pUCB30oripMB1 lacZ′ Ampr Emr3
    pMADoripE194(Ts) Emr Ampr bgaB1