TABLE 2.

Change in abundances of mRNAs for genes encoding putative glycerol-metabolizing enzymes

ComparisonGene no. (TIGR)Gene name(s) (this work)Putative gene product or functionRatio of mRNA levels
Relative gene expression levels in JH2-2 wild-type strain aerobically grown on glucose or on glycerolEF0895gldA2Glycerol dehydrogenase−3.8
EF1747glpDGlycerol-3-P dehydrogenase−1.4
EF1358gldA1Glycerol dehydrogenase−1.8
EF1360dhaKDihydroxyacetone kinase−1.7
EF1927glpFGlycerol facilitator protein+2.2
EF1928glpOGlycerol-3-P oxidase+2.9
EF1929glpKGlycerol kinase+3.1
Expression of dhaK operon relative to glpK operon in JH2-2 wild type aerobically grown:
    On glycerolEF1358, EF1360gldA1, dhaKGlycerol catabolism1.0
    On glucoseEF1358, EF1360gldA1, dhaKGlycerol catabolism+7.2
Relative gene expression levels in JH2-2 strain grown anaerobically or aerobically on glycerolEF1927, EF1928, and EF1929glpK operonGlycerol catabolism−5.23
EF1358 and EF1360dhaK operonGlycerol catabolism+21
EF0895gldA2Glycerol dehydrogenase1
EF1747glpDGlycerol-3-P dehydrogenase+2
Expression of dhaK operon relative to glpK operon in strain MMH594 aerobically grown on glycerolEF1358, EF1360dhaK operonGlycerol catabolism−2
Expression of dhaK operon relative to glpK operon in strain TX0104 aerobically grown on glycerolEF1358, EF1360dhaK operonGlycerol catabolism+263