TABLE 1.

Oligonucleotides used in this study

PrimerSequenceaSite
FNR-for1attagcatatgGCTTCGCATAATACTACACNdeI
FNR-rev2attagatgcatCAAATGGCGTGCGAGCNsiI
D148A-FGTGAAATCGTGCGCGCCCAAGGTGTTATGCTG
D148A-RCAGCATAACACCTTGGGCGCGCACGATTTCAC
1869-1attaggaattcGTACATCAGTTCGAACAGGGCGTGGEcoRI
1869-2TGACGATTTGcccgggCTTGGGCAATAGGTAAATAAGGCGGSmaI
1869-3CTATTGCCCAAGcccgggCAAATCGTCAAATAACAGGTTGCSmaI
1869-4attcagaagcttCTGAAAGGCGGTTAAGGCGGAATGGHindIII
741FORCAAATCGAAGTGGACGGGCA
741REVTGTTCGATTTTGCCGTTTCCCTG
1869rtFCAACTGGGCTTCTCCTCTGT
1869rtRATTTCGCCTTCAACGGATAC
741PfCGTCAAATAACAGGTTG
741PE1GAATCAGGGCAGTGGTCAGAG
1869FWCCAACTGCTTGATCATGCTGC
1869revGTGTGGAAATTCTTCGACAGCC
741F2TTGGCTGCGAAGGTCAGGCAG
741REV2GTATGTTCGCCCGCTATGTCG
IG1871FCATCAGCGGTTCCGTCCTTTAC
IG1871RGCGGATTTCCGGCAGAATCAG
FNR-rev1attagggatccTCAAATGGCGTGCGAGCBamHI
1869PE1GCAGCATGATCAAGCAGTTG
1869 PFTCATGGAGGCTGCAGACCAAG
1869 PR-2GCGAGCCGTCCATCATTACAG
  • a Capital letters indicate N. meningitidis-derived sequences, lowercase letters indicate sequences added for cloning purposes, and underlined letters indicate restriction enzyme recognition sites.