TABLE 3.

Sensitivity of various strains to infection by the pilus-specific phage PO4

StrainStrain sensitivity (avg PFU/ml ± SD [109])a
PAO13.96 ± 0.53
PAO1ΔchpA 3.30 ± 0.70
PAO1ΔchpA(comp)3.75 ± 0.79
PAO1ΔpilG 2.85 ± 0.72
PAO1ΔpilG(comp)4.52 ± 0.66
PAO1ΔpilB 0.00 ± 0.00*
PAO1ΔpilB(comp)3.92 ± 0.32
PAO1ΔpilA 0.00 ± 0.00*
PAO1ΔpilA(comp)4.12 ± 0.56
PAO1ΔfliC 3.92 ± 0.56
PAO1ΔfliC(comp)5.41 ± 0.14
PAO1ΔpilH 2.96 ± 0.58†
PAO1ΔpilH(comp)3.48 ± 0.45
PAO1ΔpilT CTX- pilU 0.00 ± 0.00*
PAO1ΔpilT(comp)3.39 ± 0.17
PAO1ΔpilU 2.88 ± 0.33
PAO1ΔpilU(comp)4.11 ± 0.48
PAO1ΔchpAΔpilH 4.00 ± 0.45†
PAO1ΔchpAΔpilT CTX- pilU 0.00 ± 0.00*
PAO1ΔchpAΔpilU 1.85 ± 0.30
PAO1ΔchpAΔpilTΔpilU 0.00 ± 0.00*
PAO1ΔpilGΔpilH 3.93 ± 0.72†
PAO1ΔpilGΔpilT CTX- pilU 0.00 ± 0.00*
PAO1ΔpilGΔpilU 3.07 ± 0.78
PAO1ΔpilGΔpilTΔpilU 0.00 ± 0.00*
PAO1ΔpilBΔpilH 0.00 ± 0.00*
PAO1ΔpilBΔpilT CTX- pilU 0.00 ± 0.00*
PAO1ΔpilBΔpilU 0.00 ± 0.00*
PAO1ΔpilBΔpilTΔpilU 0.00 ± 0.00*
PAO1ΔpilHΔpilT CTX- pilU 0.00 ± 0.00*
PAO1ΔpilTΔpilU 0.00 ± 0.00*
PAO1ΔpilHΔpilU 2.87 ± 0.83†
PAO1ΔpilHΔpilTΔpilU 0.00 ± 0.00*
PAO1chpA-FLAG3.45 ± 0.63
PAO1ΔchpA(AAA)-FLAG3.28 ± 0.63
PAO1pilG-His3.17 ± 0.29
PAO1pilG(D58A)-His2.99 ± 0.39
PAO1pilH-His3.31 ± 0.34
PAO1pilH(D52A)-His4.23 ± 0.23†
  • a *, No plaques were detectable even when strains were infected with 109 phage. †, The ΔpilH plaque morphology was distinguished by large zones of seemingly directional lysis and poorly defined plaques.