TABLE 3.

Sensitivity of various strains to infection by the pilus-specific phage PO4

StrainStrain sensitivity (avg PFU/ml ± SD [109])a
PAO13.96 ± 0.53
PAO1ΔchpA3.30 ± 0.70
PAO1ΔchpA(comp)3.75 ± 0.79
PAO1ΔpilG2.85 ± 0.72
PAO1ΔpilG(comp)4.52 ± 0.66
PAO1ΔpilB0.00 ± 0.00*
PAO1ΔpilB(comp)3.92 ± 0.32
PAO1ΔpilA0.00 ± 0.00*
PAO1ΔpilA(comp)4.12 ± 0.56
PAO1ΔfliC3.92 ± 0.56
PAO1ΔfliC(comp)5.41 ± 0.14
PAO1ΔpilH2.96 ± 0.58†
PAO1ΔpilH(comp)3.48 ± 0.45
PAO1ΔpilTCTX-pilU0.00 ± 0.00*
PAO1ΔpilT(comp)3.39 ± 0.17
PAO1ΔpilU2.88 ± 0.33
PAO1ΔpilU(comp)4.11 ± 0.48
PAO1ΔchpAΔpilH4.00 ± 0.45†
PAO1ΔchpAΔpilTCTX-pilU0.00 ± 0.00*
PAO1ΔchpAΔpilU1.85 ± 0.30
PAO1ΔchpAΔpilTΔpilU0.00 ± 0.00*
PAO1ΔpilGΔpilH3.93 ± 0.72†
PAO1ΔpilGΔpilTCTX-pilU0.00 ± 0.00*
PAO1ΔpilGΔpilU3.07 ± 0.78
PAO1ΔpilGΔpilTΔpilU0.00 ± 0.00*
PAO1ΔpilBΔpilH0.00 ± 0.00*
PAO1ΔpilBΔpilTCTX-pilU0.00 ± 0.00*
PAO1ΔpilBΔpilU0.00 ± 0.00*
PAO1ΔpilBΔpilTΔpilU0.00 ± 0.00*
PAO1ΔpilHΔpilTCTX-pilU0.00 ± 0.00*
PAO1ΔpilTΔpilU0.00 ± 0.00*
PAO1ΔpilHΔpilU2.87 ± 0.83†
PAO1ΔpilHΔpilTΔpilU0.00 ± 0.00*
PAO1chpA-FLAG3.45 ± 0.63
PAO1ΔchpA(AAA)-FLAG3.28 ± 0.63
PAO1pilG-His3.17 ± 0.29
PAO1pilG(D58A)-His2.99 ± 0.39
PAO1pilH-His3.31 ± 0.34
PAO1pilH(D52A)-His4.23 ± 0.23†
  • a *, No plaques were detectable even when strains were infected with 109 phage. †, The ΔpilH plaque morphology was distinguished by large zones of seemingly directional lysis and poorly defined plaques.