TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristic(s)Source or referencea
Strains
    E. coli
        MC1061K araD139 Δ(ara-leu)767 Δ(lacIPOZY) X74 rpsL galU galK rpoS::kan 26
        JM109 recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′(traD36 proAB+ lacI q lacZΔM15) 43
        BTH101F cya-99 araD139 galE15 galK16 rpsL1 (Strr) hsdR2 mcrA1 mcrB1 D. Ladant
    S. serovar Typhi
        T2Wild typeCISb
    S. serovar Typhimurium
        C52Wild type 26
        C52KC52 ΔrpoS::kan 26
        ATCC 14028Wild typeATCC
        ATCCrpoS ATCC 14028 ΔrpoS 41
        ATCCcrl ATCC 14028 Δcrl::Cm 40
        JS402Δfur-41::cat 19
        ATCCfur ATCC 14028 Δfur-41::cat
        ATCCrpoSfur ATCC 14028 ΔrpoS Δfur-41::cat
        ATCCcrl csgD-lacZ ATCC 14028 Δcrl::Cm csgD-lacZY 40
        ATCCsdhA-lacZ-kATCC 14028 sdhA-lacZY-Km 41
        ATCCfur sdhA-lacZ-kATCC 14028 Δfur-4l::cat sdhA-lacZY-Km
        ATCCcrl-lacZ ATCC 14028 crl-lacZY-Km
        ATCCrpoS crl-lacZ ATCC 14028 ΔrpoS::Cm crl-lacZY-Km
        ATCCfur crl-lacZ ATCC 14028 Δfur-41::cat crl-lacZY-Km
        ATCCrpoSfur crl-lacZ ATCC 14028 ΔrpoS Δfur-41::cat crl-lacZY-Km
        2922KATCC 14028 ΔSTM2922::Km 41
        2922Kcrl ATCC 14028 ΔSTM2922::Km Δcrl::Cm 41
        2922KrpoS ATCC 14028 ΔSTM2922::Km ΔrpoS::Cm 41
        2922KrpoS T2 ATCC 14028 ΔSTM2922::Km rpoS T2
        2922KrpoS T2 crl 2922K rpoS T2 Δcrl::Cm
        2922KkatE-lacZ 2922K Tn5B21-2.4 41
        2922KrpoS katE-lacZ 2922KrpoS Tn5B21-2.4 41
        2922Kcrl katE-lacZ 2922Kcrl Tn5B21-2.4 41
        2922KrpoS T2 katE-lacZ 2922KrpoS T2 Tn5B21-2.4
        2922KrpoS T2 crl katE-lacZ 2922K rpoS T2 Δcrl::Cm Tn5B21-2.4
Plasmids
    pACYC184Cloning vector; Cmr Tetr 6
    pACcrl-1pACYC184 with the promoterless crl gene cloned into the cat gene (crl is transcribed from the cat promoter); Tetr 40
    pACcrl Y22A pACcrl-1 with mutation Y22A in Crl
    pACcrl F35A pACcrl-1 with mutation F35A in Crl
    pACcrl C28A pACcrl-1 with mutation C28A in Crl
    pACcrl C37A pACcrl-1 with mutation C37A in Crl
    pACcrl C41A pACcrl-1 with mutation C41A in Crl
    pACcrl F53A pACcrl-1 with mutation F53A in Crl
    pACcrl W56A pACcrl-1 with mutation W56A in Crl
    pACcrl G74A pACcrl-1 with mutation G74A in Crl
    pACcrl G80A pACcrl-1 with mutation G80A in Crl
    pACcrl W82A pACcrl-1 with mutation W82A in Crl
    pACcrl F103A pACcrl-1 with mutation F103A in Crl
    pACcrl C28 -37 pACcrl-1 with mutations C28A and C37A in Crl
    pACcrl C28 -41 pACcrl-1 with mutations C28A and C41A in Crl
    pACcrl C37 -41 pACcrl-1 with mutations C37A and C41A in Crl
    pACcrl C28 -37-41 pACcrl-1 with mutations C28A, C37A, and C41A in Crl
    pUC4KSource of Km resistance cartridgePharmacia
    pSTF4 spvRAB-lacZ fusion in pQF50; Cbr 26
    pACrpoS T2 pACYC184 with a 6-kb BglII fragment carrying rpoS T2, Cmr
    pUCrpoS T2 pUC19 with a 3.3 kb SphI-ScaI fragment carrying rpoS T2, Cbr
    pUCrpoS T2KpUCrpoS T2 with STY3047::Km, Cbr Kmr
    pQE60Vector for expression of His-tagged proteins, Cbr Qiagen
    pQE30Vector for expression of His-tagged proteins, Cbr Qiagen
    pQErpoS T2 pQE60::rpoS T2 expresses a σS T2-His6 protein, Cbr
    pQE30rpoS pQE30::rpoS expresses a His6S protein, Cbr
    pQEcrl pQE30::crl expresses a His6-Crl protein, Cbr 40
    pQEcrl Y22A pQEcrl with mutation Y22A in Crl
    pQEcrl F53A pQEcrl with mutation F53A in Crl
    pQEcrl W56A pQEcrl with mutation W56A in Crl
    pQEcrl W82A pQEcrl with mutation W82A in Crl
    pKT25BACTH vector designed to express a given polypeptide fused in frame at its N-terminal end with T25 fragment; p15 ori; Kmr 23
    pKNT25BACTH vector designed to express a given polypeptide fused in frame at its C-terminal end with T25 fragment; p15 ori; Kmr 23
    pUT18BACTH vector designed to express a given polypeptide fused in frame at its C-terminal end with T18 fragment; ColE1 ori; Apr 23
    pUTCrlpUT18 expressing Crl-T18
    pUTCrlY22A pUT18 expressing Crl-T18 with mutation Y22A in Crl
    pUTCrlR51A pUT18 expressing Crl-T18 with mutation R51A in Crl
    pUTCrlE52A pUT18 expressing Crl-T18 with mutation E52A in Crl
    pUTCrlF53A pUT18 expressing Crl-T18 with mutation F53A in Crl
    pUTCrlG55A pUT18 expressing Crl-T18 with mutation G55A in Crl
    pUTCrlW56A pUT18 expressing Crl-T18 with mutation W56A in Crl
    pUTCrlW57A pUT18 expressing Crl-T18 with mutation W57A in Crl
    pUTCrlG80A pUT18 expressing Crl-T18 with mutation G80A in Crl
    pUTCrlW82A pUT18 expressing Crl-T18 with mutation W82A in Crl
    pKNTCrlpKNT25 expressing Crl-T25
    pKTRpoSpKT25 expressing T25-RpoS
    pKTRpoSΔ12 -71 pKTRpoS with in frame deletion of residues 12-71 in RpoS
    pKTRpoSΔ1 -38 pKTRpoS with deletion of residues 1-38 in RpoS
    pKTRpoSΔ1 -71 pKTRpoS with deletion of residues 1-71 in RpoS
    pKTFurpKT25 expressing T25-Fur
    pUTFurpUT18 expressing Fur-T18
  • a This study, unless otherwise noted.

  • b WHO Reference Center for Salmonella (Institut Pasteur, Paris).