TABLE 1.

E. coli strains and plasmids used in this study

Strain or plasmidDescription or genotypeSource or reference
Strains
    MG1655Wild typeE. coli Genetic Stock Center
    MJF465Frag1 ΔmscL::Cm ΔyggB ΔkefA::kan27
    MM294-StrepRStreptomycin-resistant derivative of MM294 (F glnV44 λ rfbC1? endA1 spoT1? thi-1 hsdR17 creC510)7
    MM1211MM294-StrepR aqpZ::lacZ-kan7
    NCM3306MG1655 ΔaqpZ::cam60
    WG170F lacZ trp rpsL thi Δ(putPA)101 proP21963
    WG350F lacZ trp rpsL thi Δ(putPA)101 Δ(proU)600 Δ(proP-melAB)21211
    WG269F lacZ trp rpsL thi Δ(putPA)101 proP219 Δ(proU)1 zjd-351::Tn1038
    WG980WG350 cls::Tn10dTet352
    WG1127WG350 ΔproW859::FRTThis study
    WG1128WG980 ΔproW859::FRTThis study
    WG1224WG350 ΔproP837::FRTThis study
    WG1259WG350 proP678::LumioThis study
    WG1260WG1259 cls::Tn10dTet3This study
Plasmids
    pTR10E. coli ORF mscS inserted into expression vector pBAD24This study
    pTR11pTR10 derivative that encodes MscS-CCPGCCThis study
    pTR12E. coli ORF mscL inserted into expression vector pBAD24This study
    pTR13pTR12 derivative that encodes MscL-CCPGCCThis study
    pTR14E. coli ORF aqpZ inserted into expression vector pBAD24This study
    pTR15pTR14 derivative that encodes MVCCPGCC-AqpZThis study
    pTR17pDC10 derivative that encodes MLCCPGCC-ProPThis study
    pDC288pKO3 derivative with proP678::Lumio inserted at the BamHI siteThis study