TABLE 3.

BACTH analysis of Crl interactions with σS and Fur

Coexpressed proteinsβ-Galactosidase activity (Miller units)a
T18 + T2547 ± 6
Crl-T18 + T2547 ± 6
T18 + T25-σS 43 ± 3
Crl-T18 + T25-σS 263 ± 38
Crl-T18 + T25-σS Δ1-38 455 ± 33
Crl-T18 + T25-σS Δ1-71 1830 ± 186
Crl-T18 + T25-σS Δ12-71 2352 ± 146
CrlY22A-T18 + T25-σS 44 ± 5
CrlR51A-T18 + T25-σS 59 ± 3
CrlE52A-T18 + T25-σS 102 ± 12
CrlF53A-T18 + T25-σS 48 ± 5
CrlG55A-T18 + T25-σS 138 ± 19
CrlW56A-T18 + T25-σS 44 ± 4
CrlW57A-T18 + T25-σS 74 ± 4
CrlG80A-T18 + T25-σS 205 ± 27
CrlW82A-T18 + T25-σS 55 ± 6
Crl-T18 + T25-Fur43 ± 1
Fur-T18 + Crl-T2558 ± 6
Fur-T18 + T25-Fur243 ± 32
Fur-T18 +T2555 ± 3
T18 + T25-Fur42 ± 2
T18 + Crl-T2552 ± 5
  • a The efficiencies of functional complementation between the indicated proteins were quantified by measuring β-galactosidase activities in E. coli BTH101 cells harboring the corresponding plasmids as described in Materials and Methods. β-Galactosidase activity was measured according to the method of Miller (31).