TABLE 1.

Bacterial strains, plasmids, and primers

Strain, plasmid, or primerRelevant characteristicsSource and/or reference
Strains
    PAKR1-pyocin producer, LPS A+ LPS B+ J. Lam
    PAO1R2-pyocin producerLaboratory collection
    PA14R2-pyocin producer 6
    15108/-1R5-pyocin producer, clinical isolate 11
    PAKΔR1ΔPA0620-PA0621 in R1-pyocin regionThis study
    PA14ΔR2ΔPA0620-PA0621 in R2-pyocin regionThis study
    15108/-1ΔR5ΔPA0620-PA0621 in R5-pyocin regionThis study
    PAKwbpP LPS A+ LPS B J. Lam (1)
    PAKwbpO LPS A+ LPS B J. Lam (1)
    PAKwbpL LPS A LPS B J. Lam (1)
    PAKrmlC LPS A LPS B l-Rha J. Lam (20)
    PAO1wbpM LPS A+ LPS B J. Lam (2)
    PAO1wbpL LPS A LPS B J. Lam (22)
    PAO1rmlC LPS A LPS B l-Rha J. Lam (20)
    PAO1rmd LPS A LPS B+ J. Lam (22)
    AK1012PAO1algC, LPS A LPS B, core deficient 9
Plasmids
    pEX18TSuicide vector, Tetr 8
    pRK2013RK2, helper plasmid 4
    pΔR2T42R2 pyocin region of PA14 harboring a 2,285-bp deletion encompassing PA0620 and PA0621This study
Primers
    PA0620 R5-F5′-CGATACAGGACTCATCGTGCAG-3′This study
    PA0620 R5-R5′-ACTCGGTAGCGACAATCACACA-3′This study
    PA0621-L5′-CCTCCTTGCGAGAGGTTTATGA-3′This study
    PA0621-R5′-ATCTCGATGGCGAACAACTAGG-3′This study
    PA0617-L5′-CGGCCTGGCTCATCTTAAACA-3′This study
    PA0617-R5′-ACTCGCACGGCAGACAATCC-3′This study
    PA0628-L5′-CTTCGACGTTACCTTCGACGAC-3′This study
    PA0628-R5′-CTCTCCACGGCTGGTATAGAGG-3′This study
    PA0620 R1-F5′-GCGCACATGCAGACCATATCTA-3′This study
    PA0620 R1-R5′-GGTATCGTGGTTATGCCCTGAG-3′This study
    PA0619-FD5′-CGCTTCGTGAGCTTAACGATC-3′This study
    PA0622-RD5′-GCAGGCCAGGTAGATAGACGAG-3′This study