TABLE 2.

Isolated plasmids containing linker insertions

No. isolatedPlasmidLinker position in cIPosition of stop codon or linker insertion in CIMean promoter activity (Miller units) ± SDa
PLPR
1pML3730TAA at 12NDND
1pML236TAA at 14NDND
1pML1048TAA at 18NDND
1pML1149TAA at 18NDND
1pML15162TAA at 56NDND
1pML5192TAA at 66NDND
1pML26227TAA at 78NDND
1pML3285TAA at 96NDND
1pML25317TAA at 108NDND
1pML39351TAA at 119NDND
1pML3083141 ± 15ND
1pML211960.7 ± 0.2ND
1pML193111778 ± 25ND
1pML74114892 ± 40ND
2pML17/pML1844151,095 ± 28ND
1pML2862201,081 ± 154ND
1pML3412441861 ± 163ND
2pML13/pML1422775243 ± 19ND
bpMAP150233781.3 ± 0.42.0 ± 0.1
bpMAP151257890.8 ± 0.31.5 ± 0.2
1pML8295980.6 ± 0.21.4 ± 0.1
2pML9/pML383071011.4 ± 0.110.7 ± 1
1pML353401131.1 ± 0.16.6 ± 2
1pML63581201.1 ± 0.37.7 ± 0.4
1pML323621211.3 ± 0.15.2 ± 0.3
1pML333681220.6 ± 0.12.1 ± 0.2
2pML27/pML36406/4071350.3 ± 0.11.2 ± 0.1
NonepAJ801.0 ± 0.31.3 ± 0.1
NonepCI372No cI1,023 ± 3834 ± 5
  • a A420/(ml·min·OD600). ND, not determined.

  • b —, the 15-base-pair linker was obtained not by using the GPS-LS linker scanning system but by site-directed mutagenesis.