TABLE 2.

Primers used in EMSA

PrimerSequencea
P1-F5′AAC GAATTC TTGGTACTTGTATTTAATCGTCTATC
P1-R5′AAA GGTACC GTTGTGATAACAGCACCAGCTGC3′
hla_I_F5′GTGTACAAACGAAAAAGTATCGTATGTATTTTTAATATAG3′
hla_I_R5′GTGTACATAAAATAGTTTCATTTTAATCCCCTATC3′
hla_II_F5′GTGTACAGATTACAATATAAAAATACAAATATCTTAG3′
hla_II_R5′GTACAATCTATTAGATATTTCTATGTAATGGCAAAATTTATTC3′
empF15′ACA GAATTC AATTATTTATAATGCACC3′
empR15′CCTAAGCTTTTATATAGACTCAATATTATAAC3′
eapF5′TTT GAATTC CACCATCATTATCACTCC3′
eapR5′CAT CCCGGG AAATTATCTCTCCTTTTTTG3′
vwbF15′TTC GAATTC AGATAGCGATTCGGACTC3′
vwbR15′CCTAAGCTTTAATTTTCCCTAATTAAC3′
arlR-F5′CAATAGTGAAAAGTCAGTATATGAC3′
arlR-R5′AAC GGTACC ATTTGCGTCATTTGTACACCTC3′
  • a Restriction enzyme sites are underlined.