TABLE 1.

The amounts of free 30S, 50S, and 70S ribosomes in ΔrrmJ cells expressing RrmJ or Der variants

PlasmidRibosomea (%)
70S50S30S
pINA12.355.232.5
pINARrmJ37.239.623.3
pINARrmJ + 1 mM IPTG51.623.924.4
pINAEcDer36.241.622.2
pINAEcDerGD-124.945.529.6
pINAEcDerGD-216.654.828.6
pINAEcDerGD-12NAbNANA
pINAEcDerG414R23.949.626.4
pINAEcDerG424DNANANA
pINAEcDerN469K24.548.726.9
pINAEcDerT472A26.848.225.1
  • a Values represent the average amounts of free 30S, 50S, and 70S ribosomes determined in duplicate experiments.

  • b NA, the value for 70S was not available.