TABLE 1.

Bacterial strains used in this study

Species and strainRelevant characteristicsSource
E. coli
    DH5αStratagene
    BL21 star(DE3)Invitrogen
S. aureus
    RN4220Restriction deficient, prophage cured 29
    NewmanClinical isolate, L18P substitution in SaeS 14
    ΦΝΞ-9725Strain Newman with Tn917 insertion in saeS 4
    USA300-0114Clinical isolate, no L18P substitution in SaeSNARSAa
    TB3 S. aureus Newman with three prophages deleted Δ(φNM124) 3
  • a NARSA, Network on Antimicrobial Resistance in Staphylococcus aureus.