TABLE 4.

Quantitative real-time RT-PCR in wild-type and various mutant strainsa

GeneStrainAvg copies (μg of total RNA−1)b (SD) in TV supplemented with:
GlucoseGalactoseLactose
lacCUA1592.42 × 105 (2.2 × 104)3.15 × 108 (5.0 × 107)3.24 × 108 (9.4 × 107)
lacG lacR galR mutant2.08 × 108 (6.4 × 107)NDND
lacDUA1591.95 × 105 (4.3 × 104)NDND
lacFUA1592.10 × 105 (1.5 × 104)NDND
lacEUA1598.16 × 105 (6.4 × 105)NDND
lacGUA1596.25 × 106 (9.1 × 105)2.09 × 109 (2.5 × 108)2.03 × 109 (4.1 × 108)
galKUA1591.14 × 106 (2.7 × 105)6.92 × 107 (2.3 × 107)6.96 × 106 (2.6 × 106)
lacG lacR galR mutant4.96 × 106 (6.8 × 105)NDND
galR mutant6.99 × 106 (1.0 × 106)9.30 × 106 (7.0 × 105)ND
  • a RNA was isolated from cells growing exponentially in TV supplemented with 0.5% glucose, galactose, or lactose.

  • b The data are presented as averages of results from three independent cultures. The standard deviations are indicated in parentheses. ND, not determined.