TABLE 3.

PTS activity in wild-type and selected mutant strains

StrainAvg galactose-PTS activity (nmol of NADH oxidized mg of protein−1 min−1) ± SDa
UA159184.3 ± 11
manL mutant134.0 ± 16
lacE mutant84.7 ± 11
manL lacE mutant6.4 ± 3
  • a PEP-dependent galactose-PTS activity was measured in cells that were growing exponentially in TV with 0.5% galactose (UA159 and manL and lacE mutants) or BHI supplemented with 0.5% galactose (manL lacE double mutant). Values are presented as the average results from three independent cultures.