TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionaSource or reference
Strains
    E. coli strains
        DH5αF′ endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR 44
        SM10 λpir thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmr λpir 13
    P. aeruginosa strains
        PAO1Wild type 20
        PAO1 rhlAB PAO1 with unmarked deletion in rhlAB 6
        RT101PAO1 with an rhlA-gfp fusion inserted in attB This study
        RT102PAO1 with pKD-rhlA This study
        RT103PAO1 with pKD202This study
        PAO1 rhlI PAO1 ISlacZ/hah in rhlI This study
        PAO1 pilA PAO1 ISlacZ/hah in pilA This study
        PAO1 fliM PAO1 ISlacZ/hah in fliM This study
Plasmids
    pMS402Lux reporter vector 15
    pKD-rhlA pMS402 containing rhlI promoter 15
    pKD202pMS402 containing rhlA promoter 15
    pGP003pMMB207 containing rhlI 31
    pME6032Tcr cloning vector 19
    pRT2pME6032 carrying the rhlAB geneThis study
    pMRP9-1Cbr, constitutively expresses gfp 12
  • a Cbr, carbenicillin resistance; Tcr, tetracycline resistance; Kmr, kanamycin resistance.