Influence of iron on surface motility of wild-type P. aeruginosa and an rhlAB mutant in biofilms

StrainPresence of iron% of cells showing motilitya
0-2 μm2-5 μm>5 μm
Wild type−Fe324622
rhlAB mutant−Fe77203
  • a Time-lapse experiments were conducted on 4- to 8-h-old biofilm cultures grown in flow cells in order to allow single-cell tracking. The cells were tracked over 20-min intervals, during which an image was taken every 2 min. The results are presented as percentages of cells that moved a certain distance from the total cells tracked. The data presented are averages obtained from eight different time-lapse experiments for each sample, and in each time-lapse experiment, >150 cells were tracked.