TABLE 4.

Quantitative phenotypic analysis of Caulobacter crescentus CB15 and NA1000 allele substitution strainsa

StrainSNP(s)Strain backgroundSynchrony capacityRelative attachmentMid-stationary-phase survivalRelative doubling time
nMeanSEMP value (versus CB15)P value (versus NA1000)nMeanSEMP value (versus CB15)P value (versus NA1000)nMeanSEMP value (versus CB15)P value (versus NA1000)nMeanSEMP value (versus CB15)P value (versus NA1000)
FC019CB15100.070.01<0.0001610<0.0001199.63 × 1082.4 × 1080.001191.00.01<0.0001
FC020NA1000110.710.04<0.000162.65 × 10−32.8 × 10−4<0.0001ND161.65 × 1066.8 × 1050.001160.80.01<0.0001
FC7461CB15100.090.010.341ND60.8130.1430.25ND101.30 × 1093.5 × 1080.424ND61.00.020.411ND
FC7472CB15110.110.030.264ND60.8780.0610.1ND107.27 × 1081.5 × 1080.512ND61.00.020.255ND
FC7483CB15110.110.020.104ND60.9290.1670.69ND107.92 × 1081.6 × 1080.635ND61.00.010.107ND
FC7494CB15100.080.010.593ND60.7450.1050.06ND107.87 × 1081.4 × 1080.622ND51.00.010.126ND
FC7505CB15100.040.010.04ND60.8690.1330.37ND89.56 × 1082.2 × 1080.985ND110.80.02<0.00010.676
FC7516CB15110.110.020.169ND60.8630.110.27ND102.06 × 1095.5 × 1080.045ND91.00.010.051ND
FC7527CB15110.110.030.222ND60.8710.1080.28ND82.02 × 1098.3 × 1080.114ND61.00.011ND
FC7538CB15110.070.010.6ND60.9890.1130.93ND173.10 × 1078.3 × 1060.0010.00291.00.010.554ND
FC7549CB15110.070.010.966ND62.39 × 10−32.4 × 10−4<0.00010.50381.93 × 1096.7 × 1080.102ND61.00.020.025ND
FC75510CB15110.080.010.52ND61.140.1750.46ND176.58 × 1071.3 × 1070.001<0.000171.00.010.065ND
FC7561NA1000110.720.03ND0.7762.63 × 10−31.6 × 10−4ND0.9791.48 × 1063.0 × 105ND0.854150.80.01ND0.271
FC7572NA1000110.810.02ND0.0462.72 × 10−32.7 × 10−4ND0.86115.62 × 1062.3 × 106ND0.064130.80.01ND0.240
FC7583NA1000110.790.02ND0.0962.77 × 10−32.1 × 10−4ND0.72784.13 × 1062.0 × 106ND0.16060.80.01ND0.438
FC7594NA1000110.740.02ND0.5362.66 × 10−32.0 × 10−4ND0.96482.43 × 1068.4 × 105ND0.50060.80.01ND0.674
FC7605NA1000100.750.03ND0.4862.69 × 10−32.6 × 10−4ND0.90283.1 × 1061.1 × 106ND0.257121.00.010.065<0.0001
FC7616NA1000110.830.03ND0.0263.37 × 10−33.6 × 10−4ND0.14483.27 × 1061.2 × 106ND0.21060.90.01ND0.024
FC7627NA1000110.750.02ND0.3862.87 × 10−32.8 × 10−4ND0.58982.78 × 1061.3 × 106ND0.41360.80.01ND0.337
FC7638NA1000110.740.02ND0.4962.93 × 10−32.8 × 10−4ND0.492171.39 × 1074.6 × 1060.0010.01670.80.02ND0.593
FC7649NA1000110.780.02ND0.1660.9010.1250.46<0.000183.96 × 1061.1 × 106ND0.07960.80.01ND0.165
FC76510NA1000100.810.03ND0.0662.92 × 10−33.1 × 10−4ND0.53172.09 × 1074.3 × 1060.001070.80.03ND0.952
FC766NA (large indel)NA1000100.070.020.793<0.000162.77 × 10−32.2 × 10−4ND0.73182.65 × 1068.3 × 105ND0.39060.80.01ND0.243
FC10498 and 10CB15NDNDNDNDNDNDNDNDNDND84.93 × 1062.2 × 1060.0180.081NDNDNDNDND
FC10508 and 10NA1000NDNDNDNDNDNDNDNDNDND81.58 × 1096.3 × 1080.2730.002NDNDNDNDND
  • a For graphical representation of these data, see Fig. 5. Synchrony capacity is defined as ODs/(ODs + ODp). Attachment is measured relative to that of CB15. Mid-stationary-phase survival is measured as the number of CFU/ml after 96 h of growth in liquid culture. Doubling times are measured relative to that of CB15. P values were calculated using unpaired two-tailed t tests to compare the phenotype of a strain with that of its parent and, only if significantly different, with that of the other parent. n, number of replicates; NA, not applicable; ND, not done.