TABLE 2.

Plasmids used and effector knockout vector collection generated in this work

PlasmidDescriptionAntibiotic resistanceSource or reference
pGEM-TCloning vectorAmprPromega
pKD4pANTSγ derivative containing an FRT-flanked kanamycin resistance geneAmpr Kmr11
pFLP2Contains a flipase geneAmpr19
pGEM-T-KmFRT EcoRIpGEM-T derivative containing a Km resistance gene flanked by FRT and EcoRI sitesAmpr KmrThis work
pGEM-T-KmFRT BamHIpGEM-T derivative containing a Km resistance gene flanked by FRT and BamHI sitesAmpr KmrThis work
pGEM-T-KmFRT XhoIpGEM-T derivative containing a Km resistance gene flanked by FRT and XhoI sitesAmpr KmrThis work
pAZJ6pGEM-T derivative carrying the ΔavrB2::KmFRT knockout alleleAmpr KmrThis work
pAZJ7pGEM-T derivative carrying the ΔhopR1::KmFRT knockout alleleAmpr KmrThis work
pAZJ8pGEM-T derivative carrying the ΔavrB4-1::KmFRT knockout alleleAmpr KmrThis work
pAZJ9pGEM-T derivative carrying the ΔhopW1-1::KmFRT knockout alleleAmpr KmrThis work
pAZJ10pGEM-T derivative carrying the ΔhopX1::KmFRT knockout alleleAmpr KmrThis work
pAZJ11pGEM-T derivative carrying the ΔhopAE1::KmFRT knockout alleleAmpr KmrThis work
pAZJ12pGEM-T derivative carrying the ΔhopAB1::KmFRT knockout alleleAmpr KmrThis work
pAZJ13pGEM-T derivative carrying the ΔhopAW1::KmFRT knockout alleleAmpr KmrThis work
pAZJ14pGEM-T derivative carrying the ΔavrRps4::KmFRT knockout alleleAmpr KmrThis work
pAZJ15pGEM-T derivative carrying the ΔhopG1::KmFRT knockout alleleAmpr KmrThis work
pAZJ16pGEM-T derivative carrying the ΔhopQ1::KmFRT knockout alleleAmpr KmrThis work
pAZJ17pGEM-T derivative carrying the ΔhopD1::KmFRT knockout alleleAmpr KmrThis work
pAZJ18pGEM-T derivative carrying the ΔhopI1::KmFRT knockout alleleAmpr KmrThis work
pAZJ19pGEM-T derivative carrying the ΔhopAV1::KmFRT knockout alleleAmpr KmrThis work
pAZJ20pGEM-T derivative carrying the ΔhopAT1::KmFRT knockout alleleAmpr KmrThis work
pAZJ21pGEM-T derivative carrying the ΔhopAF1::KmFRT knockout alleleAmpr KmrThis work
pAZJ22pGEM-T derivative carrying the ΔhopAS1::KmFRT knockout alleleAmpr KmrThis work
pAZJ23pGEM-T derivative carrying the ΔhopW1-2::KmFRT knockout alleleAmpr KmrThis work
pAZJ24pGEM-T derivative carrying the ΔavrD1::KmFRT knockout alleleAmpr KmrThis work
pAZJ25pGEM-T derivative carrying the ΔhopAU1::KmFRT knockout alleleAmpr KmrThis work
pAZJ26pGEM-T derivative carrying the ΔhopAK1::KmFRT knockout alleleAmpr KmrThis work
pAZJ27pGEM-T derivative carrying the ΔavrB4-2::KmFRT knockout alleleAmpr KmrThis work
pAZJ28pGEM-T derivative carrying the ΔhopAJ1::KmFRT knockout alleleAmpr KmrThis work
pAZJ29pGEM-T derivative carrying the ΔhopF3::KmFRT knockout alleleAmpr KmrThis work
pAME8pPnptII::avrRpt2Ampr Kmr30
pAMEXVector for expression of N-terminal protein fusions to AvrRpt281-255 under a PnptII promoterAmpr Kmr30
pAME9.18pAMEX derivative expressing the HopW1-1::′AvrRpt2 protein fusionAmpr KmrThis work
pAME9.19pAMEX derivative expressing the AvrB4-2::′AvrRpt2 protein fusionAmpr KmrThis work
pAME9.20pAMEX derivative expressing the PSPPH1154::′AvrRpt2 protein fusionAmpr KmrThis work
pAME9.21pAMEX derivative expressing the avrE1::′AvrRpt2 protein fusionAmpr KmrThis work
pJRU1pPlac::hopW1-1/2Ampr KmrThis work
pJRU2pPlac::avrB4-2Ampr KmrThis work
pJRU3pPnptII::avrB4-1Ampr KmrThis work
pJRU4pPnptII::avrB4-2Ampr KmrThis work
pJRU5pavrB4-1Ampr KmrThis work
pJRU6pPnptII::hopW1-1/2Ampr KmrThis work