TABLE 2.

Transfer frequencies of CTn341 mutant derivatives and complemented strains

AssayaDonorbComplementing PlasmidRecipientTransfer Frequency
Mating out
    1BTSpFD699 (wt)NoneDH10B4.4 × 10−4
    2BT-Jo36 (mobB341)NoneDH10B<10−9
    3BT-Jo36 (mobB341)pMobDH10B1.9 × 10−4
    4BT-Jo36 (mobB341)pMobABDH10B1.1 × 10−4
Bacteroides-Bacteroides matings
    5IB255 (wt)NoneIB1323.7 × 10−4
    6IB255 (wt)pMobCΔNIB1325.9 × 10−5
    7BF-Jo82 (mobC341)NoneIB1321.7 × 10−6
    8BF-Jo82 (mobC341)pMobCΔNIB1328.6 × 10−8
    9IB255 (wt)pMobCΔNNonec<10−9
    10BF-Jo82 (mobC341)pMobCΔNNone<10−9
  • a Mating-out assays measure transfer of CTn341 mutant derivatives from Bacteroides to E. coli recipients. Bacteroides-Bacteroides matings measure transfer of CTn341 mutant derivatives from B. fragilis donors to B. fragilis recipient IB132 (Fur).

  • b The specific mutation or wild-type (wt) status of the donor strain is given in parentheses next to the strain number.

  • c Matings with no recipients were performed to determine the level of background conversion of the donor strain to fusidic acid resistance.