TABLE 3.

Vectors and plasmids

PlasmidGenotypePrimer pair(s) used for cloningReference or source
pAC6bla lacZ cat amyE′…′amyE54
pGEMcatori(f1) lacZ′(MCS) bla cat63
pET-16bT7 lac promoter, His10 tag, MCS, T7 terminator; lacI pBR322-Ori blaNovagen
pMADerm ori(pE194-Ts) MCS-PclpB-bgaB ori(pBR322) bla2
pAJu604pAC6 PydhE(−74)-lacZTM1395/TM0480This study
pDW103pMAD PliaI-liaIH up/down overlapTM1056/TM1057, TM1055/TM1058This study
pDW105pMAD liaR-terminator-gerAC overlapTM1184/TM1185, TM1186/TM1187This study
pDW602pAC6 PydhE(−96)-lacZTM1559/TM0480This study
pDW604pAC6 PydhE(−71)-lacZTM1583/TM0480This study
pDW1604pET16b-liaRTM1045/TM0124This study
pFK1601pET16b-liaHTM0118/TM0120This study
pJR601pAC6 PydhE(-57)-lacZTM0682/TM0480This study
pJR602pAC6 PydhE(-34)-lacZTM0683/TM0480This study
pSJ102pMAD liaF up/down overlapTM0457/TM0574, TM0575/TM0458This study
pSK602pAC6 PydhE-lacZTM0480/TM0481This study