TABLE 2.

Primers used in this work

No.Primer nameSequence (5′ → 3′)
1sacB FwdATATCCCGGGCCATGGCCATCACATATACCTGCCGTTC
2sacB RvsATATCCCGGGCCATGGTTATTTGTTAACTGTTAATTGTCCTTG
3tatABC FwdATATGAATTCTGCGCGAGACGCGCGCGA
4tatABC RvsATATAAGCTTCTCCTTCGACGCAGCATTGCG
55′ upstream tat FwdATATGCGGCCGCGGTTTCAGATGCGCGTAACG
65′ upstream tat RvsATATCCCGGGGTATGGCCAGCGTGTCGGC
73′ downstream tat FwdATATCCCGGGACCGCGGAGTTCTTGCGCC
83′ downstream tat RvsATATGGTACCCGACATTTGCGCTTTCGTCCG
9tatC FwdATATGAATTCCGCCGGCTGCGAATAAG
10tatC RvsATATGAATTCGCTGCATGACATCGGAG
11tat confirmation FwdCACCGAAGCCTGAAGAAGAC
12Tat confirmation RvsGCTCCTTCGAAGTCCATCAG
13nptII out LeftTTCGGAATCGTTTTCCGGGAG
14nptII out RightTTAGCAGCCCTTGCGCCCTG