TABLE 2.

Anaerobic purification of PduS-His6

Purification stepTotal protein (mg)Cob(III)alamin reductaseCob(II)alamin reductasea
Sp act (μmol min−1 mg−1)Total activity (μmol min−1)Purification (fold)Sp act (nmol min−1 mg−1)Total activity (nmol min−1)Purification (fold)
Whole-cell extract2203.9 ± 0.385814.4 ± 0.49681
Soluble fraction1664.2 ± 0.46971.14.9 ± 0.58131.1
Ni-NTA eluate1.642.3 ± 3.267.710.854.5 ± 4.287.212.4
  • a Iodoacetate at 0.5 mM was used to trap produced cob(I)alamin.