TABLE 4.

Two-hybrid analysis of PduS-PduO interactions

Plasmid pairNo. of coloniesa
Nonselective mediumSelective medium (3-AT)
1/1001/1,0001/101/1,000
pBT-LGF2/pTRG-Gal11bTNTCc∼2,000TNTC∼2,000
pBT-pduS/pTRG-pduO>2,000255>2,000152
pBT-pduO/pTRG-pduS>2,000296>2,000129
pBT-pduS/pTRG>2,00041000
pBT/pTRG-pduS>2,00056410
pBT-pduO/pTRG>2,00017020
pBT/pTRG-pduO>2,00040030
  • a Number of colonies formed following cotransformation of the reporter strain with the bait and target plasmids on selective and nonselective media at the dilution indicated.

  • b LGF2 and Gal11 are positive controls known to strongly interact.

  • c TNTC, too numerous to count.