TABLE 1.

Strains and plasmids used in this study

Strain (GenBank accession no.) or plasmidRelevant characteristic(s)Source or reference
Strains
E. faecalis
    OG1RFWild-type strain, Fusr Rifr, p-Cl-Pher34
    OG1RF Tn libraryOG1RF Tn insertion mutants17
    TX5608 (ΔebpABC mutant)OG1RF ebpABC deletion mutant35
    ΔrnjB mutantOG1RF rnjB in-frame deletionThis study
E. coli
    XL1-BlueCloning hostStratagene
    EC1000Cloning host, provides RepA in trans25
Plasmids
    pQE30:rEbpCRecombinant EbpC expression vector35
    pMSP3535Nisin-inducible expression vector, Emr8
    pCJK47Delivery plasmid with pheS* counterselectable marker8
    pMSP:rnjBrnjB cloned into pMSP3535This study
    pTEX5585PebpA::lacZ fusion in pTCV-lacZ6
    pTEX5586PebpR::lacZ fusion in pTCV-lacZ5
    pCJK47:dapAIntermediate construct carrying dapA-flanking sequenceThis study
    pCJK47:rnjBIntermediate construct carrying rnjB-flanking sequenceThis study