TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
Strains
    CM1000DJ480 ΔdsrA14NM308 + P1(NM14)
    CM1001DJ480 ΔdsrA14 rpoS*NM307 + P1(NM14)
    CM1010DJ480 ΔdsrA14 rne-3071 zce-726::Tn10CM1000 + P1 (EM1277)
    CM1050DJ480 ΔdsrA14 Δrnc-1223::catCM1000 + P1(CM1223)
    CM1052DJ480 ΔdsrA14 rne-3071 zce-726::Tn10 Δrnc-1223::catCM1010 + P1(CM1223)
    CM1060DJ480 ΔdsrA14 Δara714CM1000 + P1(LMG194), P1(EM1448)
    CM1061DJ480 ΔdsrA14 Δara714 rpoS*CM1001 + P1(LMG194), P1(EM1448)
    CM1062DJ480 ΔdsrA14 rprA1::kan Δara714CM1060 + P1(NM22521)
    CM1063DJ480 ΔdsrA14 rprA1::kan rpoS* Δara714CM1061 + P1(NM22521)
    CM1064DJ480 ΔdsrA14 rne-3071 zce-726::Tn10 Δara714CM1060 + P1(EM1277)
    CM1080DJ480 ΔdsrA14 Δara714 Δrnc-1223::catCM1060 + P1(CM1223)
    CM1082DJ480 ΔdsrA14 rprA1::kan Δara714 Δrnc-1223::catCM1062 + P1(CM1223)
    CM1083DJ480 ΔdsrA14 rprA1::kan rpoS* Δara714 Δrnc-1223::catCM1063 + P1(CM1223)
    CM1086DJ480 ΔdsrA14 rne-3071 zce-726::Tn10 Δrnc-1223::cat Δara714CM1064 + P1(CM1223)
    CM1223NM300 Δrnc-1223::catThis study
    DJ480MG1655 ΔlacX747
    DY330W3110 ΔlacU169 gal-490λcI857 Δ(cro-bioA)63
    EM1277DJ480 rne-3071 zce-726::Tn1031
    EM1448DJ480 Δara714 leu+Lab strain
    JNB001DJ480 ΔdsrA14 rprA1::kanCM1000 + P1(NM22521)
    JNB002DJ480 ΔdsrA14 rprA1::kan rpoS*CM1001 + P1(NM22521)
    LMG194Δara714 leu::Tn1018
    MG1655Wild typeLab strain
    NC397HME45 lacI′<>kan-Ter<>cat-sacB<>lacZYA51
    NM1DY330 ΔdsrA14::cat-sacBThis study
    NM14DY330 ΔdsrA14This study
    NM22521C600 rprA1::kanLab strain
    NM300DJ480 mini-λ tet54
    NM301NM300 rpoS*This study
    NM307NM301 rpoS* ΔdsrA::cat-sacB his::Tn10NM301 + P1(NM311)
    NM308DJ480 ΔdsrA::cat-sacB his::Tn10DJ480 + P1(NM311)
    NM111Hfr H ΔdsrA::cat-sacB his::Tn10SG2204 +P1(NM1)
    SG2204Hfr H his::Tn1056
Plasmids
    pACS21pBR322 rnc+53
    pNM12pBAD24 derivative28
    pNM13pBAD-dsrA+28
    pNM33pBAD-dsrA*28
    pNM100pBAD-rprA+29
    pNM109pBAD-rprA*29