TABLE 1.

E. coli strains and plasmids used in this study

NameGenotype or main characteristicsAntibiotic resistanceReference
Strains
    JW2669BW25113 ΔrecA::kanKmr2
    MFDpirMG1655 RP4-2-Tc::[ΔMu1::aac(3)IVaphAnic35-ΔMu2::zeo] ΔdapA::(erm-pir) ΔrecAAprar Zeor ErmrThis study
    MG1655K-12 wild-type strain6
    MG1655-sSpontaneous streptomycin-resistant mutant of MG1655Laboratory collection
    MG1655 ΔlacMG1655 ΔlacIZ::cat LacCmrThis study
    MG1655-s ΔlacMG1655-s ΔlacIZ::frt LacSmrThis study
    S17-1 λpir(F) RP4-2-Tc::Mu aphA::Tn7recAλpirlysogenSmr Tpr40
    SM10 λpir(F) RP4-2-Tc::Mu recA λpir lysogenKmr40
    ω7249(F) RP4-2-Tc::Mu Δnic35 ΔdapA::(erm-pir)Kmr Ermr3
Plasmids
    pKD3Source of the cat::FRT cassetteApr Cmr12
    pKOBEGoriR101ts araC arabinose-inducible λred γβα operonCmr9
    pKOBEGApKOBEG derivativeApr9
    pSC189oriT Π-dependent ori R6K mariner-based transposon TnSC189Kmr Apr11
    pSC189-CmLike pSC189 but carries TnSC189 Δkan::catCmr AprF. Leroux
    pCP20Rep(Ts) Flp+Cmr Apr10