TABLE 2.

List of M. smegmatis strains used in the study

M. smegmatis strainRelevant characteristic(s)Reference
SN2Laboratory strain23
mc2155 or wild typeHigh-efficiency transformation strain31
WT(L5 att::pMV361)mc2155 harboring an integrative vector, pMV361 (Hygr), at the L5 att site of the chromosomeThis study
ung::kan strainmc2155 with ung gene disrupted with kan cassette36
ung::kan(L5 att::narK2ung) strainung::kan strain harboring the integrative vector, pMVnarK2ung (Hygr), at the L5 att site of the chromosomeThis study
WT(L5 att::narK2ung)mc2155 strain harboring pMVnarK2ung at the L5 att site of the chromosome