TABLE 1.

Spontaneous suppressor mutations that accumulated in ΔackA mutants

Phenotype group and mutated geneMutation in independent isolates
Mucoid large colony suppressors
    spxBPhe269Leu (TTT to CTT)
    spxBFrameshift mutation ΔC in codon 264
    spxBGly472Asp (GGC to GAC)
    spxBChange in mapped transcription start from G to T
Rough medium-sized colony suppressors
    spxBTrp339STOP (TGG to TAG)
    cps2EaFrameshift mutation ΔA in codon 326 (in run of 7 As)
    spxBGly423Cys (GGT to TGT)
    cps2EaFrameshift mutation ΔA in codon 326 (in run of 7 As)
    spxRFrameshift mutation ΔG in codon 115 (in run of 5 Gs)
    cps2EaFrameshift mutation ΔA in codon 326 (in run of 7 As)
Additional capsule mutations identified in rough medium-sized colony suppressors
    cps2EFrameshift mutation ΔG in codon 111
    cps2EArg347Cys (CGT to TGT)
    cps2EFrameshift mutation ΔA in codon 419
    cps2EInsertion of T after codon 204
    cps2CInsertion of A after codon 45
    cps2CInsertion of T in codon 113
  • a Isolated three times from separate independent transformations.