ClO2 preferentially kills cells mutant for matrix productiona

CoculturebCell ratioc
In untreated cultureAfter treatment
3610/ΔepsH mutant0.726.4
3610/Δspo0A mutant0.909.9
  • a The experiment assumes that, in shaking culture, the EPS produced by wild-type cells did not provide protection to mutant cells in trans.

  • b lacZ-bearing 3610 cells were mixed with unlabeled mutant cells, and the mixture was grown to exponential phase and then split in two, with one portion being treated with ClO2 (16 μg/ml) and the other portion being left untreated.

  • c Determined by measuring the numbers of wild-type and mutant CFU.